Our story on coronavirus research

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  • Honrubia, J. M., Gutierrez-Álvarez, J., Sanz-Bravo, A., González-Miranda, E., Muñoz-Santos, D., Castaño-Rodriguez, C., Wang, L., Villarejo-Torres, M., Ripoll-Gómez, J., Esteban, A., Fernandez-Delgado, R., Sánchez-Cordón, P. J., Oliveros, J. C., Perlman, S., McCray, P. B. Jr., Sola, I., Enjuanes, L. 2023. SARS-CoV-2-Mediated lung edema and replication are diminished by cystic fibrosis transmembrane conductance regulator modulators. mBio, 14, e0313622.
  • Bello-Perez, M., Hurtado-Tamayo, J., Requena-Platek, R., Canton, J., Sánchez-Cordón, P. J., Fernandez-Delgado, R., Enjuanes, L., Sola, I. 2022. MERS-CoV ORF4b is a virulence factor involved in the inflammatory pathology induced in the lungs of mice. PLoS Pathog. 18, e1010834.
  • Enjuanes, L., Sola, I., Zuñiga, S., Honrubia, J. M., Bello-Perez, M., Sanz-Bravo, A., Gonzalez-Miranda, E., Hurtado-Tamayo, J., Requena-Platek, R., Wang, L., Muñoz-Santos, D., Sanchez, C. M., Esteban, A., Ripoll-Gomez, J. 2022. Nature of viruses and pandemics: Coronaviruses. Current Research in Immunology, 3, 151-158.
  • Wong, L. R., Zheng, J., Wilhelmsen, K., Li, K., Ortiz, M. E., Schnicker, N. J., Thurman, A., Pezzulo, A. A., Szachowicz, P. J., Li, P., Pan, R., Klumpp, K., Aswad, F., Rebo, J., Narumiya, S., Murakami, M., Zuniga, S., Sola, I., Enjuanes, L., Meyerholz, D. K., Fortney, K., McCray, P. B., Jr., Perlman, S. 2022. Eicosanoid signaling blockade protects middle-aged mice from severe COVID-19. Nature, 605,146-151.
  • Morales, L., Oliveros, J. C., Enjuanes, L., Sola, I., 2022. Contribution of host miRNA-223-3p to SARS-CoV-induced lung inflammatory pathology. mBio 13, e0313521.
  • Gutierrez-Álvarez, J., Honrubia, J. M., Sanz-Bravo, A., Gonzalez-Miranda, E., Fernández-Delgado, R., Rejas, M. T., Zúñiga, S., Sola, I., Enjuanes, L. 2021. Middle East respiratory syndrome coronavirus vaccine based on a propagation-defective RNA replicon elicited sterilizing immunity in mice. Natl. Acad. Sci. USA 118, e2111075118.
  • Gutierrez-Alvarez, J., Wang, L., Fernandez-Delgado, R., Li, K., McCray, P.B., Jr., Perlman, S., Sola, I., Zuniga, S., Enjuanes, L., 2021. Middle East Respiratory Syndrome Coronavirus Gene 5 Modulates Pathogenesis in Mice.Virol. 95, e01172-01120.
  • Gorbalenya, A. E., Baker, S. C., Baric, R. S., de Groot, R. J., Drosten, C., Gulyaeva, A. A., Haagmans, B. L., Lauber, C., Leontovich, A. M., Neuman, B. W., Penzar, D., Perlman, S., Poon, L. L. M., Samborskiy, D. V., Sidorov, I. A., Sola, I., Ziebuhr, J. 2020. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2 Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. Microbiol. 5, 536–544
  • Castaño-Rodriguez, C., Honrubia, J. M., Gutiérrez-Álvarez, J., DeDiego, M. L., Nieto-Torres, J. L., Jimenez-Guardeño, J. M., Regla-Nava, J. A., Fernandez-Delgado, R., Verdia-Báguena, C., Queralt-Martín, M., Kochan, G., Perlman, S., Aguilella, V. M., Sola, I., Enjuanes, L. 2018. Role of severe acute respiratory syndrome coronavirus viroporins E, 3a, and 8a in replication and pathogenesis. mBio 9, e02325-17.
  • Canton, J., Fehr, A. R., Fernandez-Delgado, R., Gutierrez-Alvarez, F. J., Sanchez-Aparicio, M. T., Garcia-Sastre, A., Perlman, S., Enjuanes, L., Sola, I., 2018. MERS-CoV 4b protein interferes with the NF-kappaB-dependent innate immune response during infection. PLoS Pathog. 14, e1006838.
  • Enjuanes, L., Zuniga, S., Castano-Rodriguez, C., Gutierrez-Alvarez, J., Canton, J., Sola, I., 2016. Molecular basis of coronavirus virulence and vaccine development. Adv. Virus Res. 96, 245-286.
  • Sola, I., Almazán, F., Zúñiga, S., Enjuanes, L., 2015. Continuous and discontinuous RNA synthesis in coronaviruses. Annu. Rev. Virol. 2, 265-288.
  • Nieto-Torres, J. L., Verdia-Baguena, C., Jimenez-Guardeno, J. M., Regla-Nava, J. A., Castano-Rodriguez, C., Fernandez-Delgado, R., Torres, J., Aguilella, V. M., Enjuanes, L., 2015. Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome. Virology 485, 330-339.
  • Jimenez-Guardeno, J. M., Nieto-Torres, J. L., DeDiego, M. L., Regla-Nava, J. A., Fernandez-Delgado, R., Castano-Rodriguez, C., Enjuanes, L. 2014. The PDZ-binding motif of severe acute respiratory syndrome coronavirus envelope protein Is a determinant of viral pathogenesis. PLoS Pathog. 10, e1004320.
  • Nieto-Torres, J.L., DeDiego, M.L., Verdia-Baguena, C., Jimenez-Guardeno, J.M., Regla-Nava, J.A., Fernandez-Delgado, R., Castano-Rodriguez, C., Alcaraz, A., Torres, J., Aguilella, V.M., Enjuanes, L. 2014. Severe acute respiratory syndrome coronavirus envelope protein ion channel activity promotes virus fitness and pathogenesis. PLoS Pathog. 10, e1004077.
  • DeDiego, M. L., Nieto-Torres, J. L., Regla-Nava, J. A., Jimenez-Guardeno, J. M., Fernandez-Delgado, R., Fett, C., Castaño-Rodriguez, C., Perlman, S., Enjuanes, L. 2014. Inhibition of NF-kappaB mediated inflammation in severe acute respiratory syndome coronavirus-infected mice increases survival. Virol. 88, 913-924.
  • Almazan, F., DeDiego, M.L., Sola, I., Zuniga, S., Nieto-Torres, J.L., Marquez- Jurado, S., Andres, G., Enjuanes, L. 2013. Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate. mBio 4, e00650-00613.
  • Morales, L., Oliveros, J. C., Fernandez-Delgado, R., tenOever, B. R., Enjuanes, L., Sola, I., 2017. SARS-CoV-encoded small RNAs contribute to infection-associated lung pathology. Cell Host Microbe 21, 344-355.
  • DeDiego, M. L., Nieto-Torres, J. L., Jimenez-Guardeno, J. M., Regla-Nava, J. A., Alvarez, E., Oliveros, J. C., Zhao, J., Fett, C., Perlman, S., Enjuanes, L. 2011. Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis. PLoS Pathog. 7, e1002315.
  • Sola, I., Mateos-Gomez, P. A., Almazan, F., Zuñiga, S., and Enjuanes, L. 2011. RNA-RNA and RNA-protein interactions in coronavirus replication and transcription. RNA Biol. 8, 237-248.
  • Nieto-Torres, J. L., DeDiego, M. L., Alvarez, E., Jimenez-Guardeno, J. M., Regla-Nava, J. A., Llorente, M., Kremer, L., Shuo, S., and Enjuanes, L. 2011. Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein. Virology 415, 69-82.
  • Zuñiga, S., Cruz, J. L. G., Sola, I., Mateos, P. A., Palacio, L., and Enjuanes, L. 2010. Coronavirus nucleocapsid protein facilitates template switching and is required for efficient transcription. Virol. 84, 2169-2175.
  • DeDiego, M. L., Alvarez, E., Almazan, F., Rejas, M. T., Lamirande, E., Roberts, A., Shieh, W. J., Zaki. S. R., Subbarao, K., and Enjuanes, L. 2007. A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo. Virol. 81, 1701-1713.
  • Gorbalenya, A. E., Enjuanes, L., Ziebuhr, J., and Snijder, E. J. 2006. Nidovirales: Evolving the largest RNA virus genome. Virus Res. 117, 17-37.
  • Enjuanes, L., Almazan, F., Sola, I., and Zuñiga, S. 2006. Biochemical aspects of coronavirus replication and virus host-interaction. Ann. Rev. Microbiol. 60, 211-230.
  • Zuñiga, S., Sola, I., Alonso, S., Enjuanes, L. 2004. Sequence motifs involved in the regulation of discontinuous coronavirus subgenomic RNA synthesis. Virol. 78, 980-994.
  • Holmes, K. V., Enjuanes, L. 2003. The SARS coronavirus: a postgenomic era. Science 300, 1377-1378.
  • Ortego, J., Escors, D., H. Laude y L. Enjuanes. Generation of a replication-competent, propagation-deficient virus vector based on transmissible gastroenteritis coronavirus genome. J. Virol. 76, 11518-11529.
  • Almazan, F., Gonzalez, J. M., Penzes, Z., Izeta, A., Calvo, E., Plana-Duran, J., Enjuanes, L. 2000. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Nat. Acad. Sci. USA. 97, 5516-5521.
  • Castilla, J., Pintado, B., Sola, I., Sanchez-Morgado, J. M., and Enjuanes, L. 1998. Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk. Nature Biotech. 16, 349-354
  • Alhammad, Y. M., Parthasarathy, S., Ghimire, R., O’Connor, J. J., Kerr, C. M., Pfannenstiel, J. J., Chanda, D., Miller, C. A., Unckless, R. L., Zuniga, S., Enjuanes, L., More, S., Channappanavar, R., Fehr, A. R. 2023. SARS-CoV-2 Mac1 is required for IFN antagonism and efficient virus replication in mice. bioRxiv, doi: 10.1101/2023.1104.1106.535927.
  • Hussein, M., Andrade Dos Ramos, Z., Vink, M. A., Kroon, P., Yu, Z., Enjuanes, L., Zuñiga, S., Berkhout, B., Herrera-Carrillo, E. 2023. Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2. Viruses, 15, 686
  • Myeni, S. K., Bredenbeek, P. J., Knaap, R. C. M., Dalebout, T. J., Morales, S. T., Sidorov, I. A., Linger, M. E., Oreshkova, N., van Zanen-Gerhardt, S., Zander, S. A. L., Enjuanes, L., Sola, I., Snijder, E. J., Kikkert, M., 2023. Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase. Nat. Commun. 14, 1141.
  • Somovilla, P., García-Crespo, C., Martínez-González, B., Soria, M. E., de Ávila, A. I., Gallego, I., Mínguez, P., Durán-Pastor, A., Ferrer-Orta, C., Salar-Vidal, L., Esteban-Muñoz, M., Zuñiga, S., Sola, I., Enjuanes, L., Esteban, J., Fernandez-Roblas, R., Gadea, I., Gómez, J., Verdaguer, N., Domingo, E., Perales, C. 2023. Atypical mutational spectrum of SARS-CoV-2 replicating in the presence of ribavirin. Antimicrob. Agents Chemother. 67, e0131522.
  • Honrubia, J. M., Gutierrez-Álvarez, J., Sanz-Bravo, A., González-Miranda, E., Muñoz-Santos, D., Castaño-Rodriguez, C., Wang, L., Villarejo-Torres, M., Ripoll-Gómez, J., Esteban, A., Fernandez-Delgado, R., Sánchez-Cordón, P. J., Oliveros, J. C., Perlman, S., McCray, P. B. Jr., Sola, I., Enjuanes, L. 2023. SARS-CoV-2-Mediated lung edema and replication are diminished by cystic fibrosis transmembrane conductance regulator modulators. mBio, 14, e0313622.
  • Widjaja, I., Wang, C., van Haperen, R., Gutierrez-Alvarez, J., van Dieren, B., Raj, V. S., Li, W., Sola, I., Grosveld, F. G., van Kuppeveld, F. J. M., Haagmans, B. L., Enjuanes, L., Drabek, D., Bosch, B. J. 2022. Protective human monoclonal antibodies against Middle-East respiratory syndrome coronavirus that target multiple functions of the virus envelope spike glycoprotein. Under Submission.
  • Wang, L., Guzman, M., Isabel Sola, I., Enjuanes, L., Zuñiga, S. 2022. Cytoplasmic ribonucleoprotein complexes, RNA helicases and coronavirus infection. Frontiers in Virology. In press.
  • Bello-Perez, M., Esparza, I., De la Encina, A., Bartolome, T., Molina, T., Sanjuan, E., Falco, A., Enjuanes, L., Sola, I., Usera, F., 2022. Pulsed-Xenon Ultraviolet Light Highly Inactivates Human Coronaviruses on Solid Surfaces, Particularly SARS-CoV-2. J. Environ. Res. Public Health, 19, 13780.
  • Padilla-Blanco, M., Vega, S., Enjuanes, L., Morey, A., Lorenzo, T., Marín, C., Ivorra, C., Maiques, E., Rubio, V., Rubio-Guerri, C. 2022. Detection of SARS-CoV-2 in a dog with hemorrhagic diarrhea. BMC Vet Res. 18, 370.
  • Bello-Perez, M., Hurtado-Tamayo, J., Requena-Platek, R., Canton, J., Sánchez-Cordón, P. J., Fernandez-Delgado, R., Enjuanes, L., Sola, I. 2022. MERS-CoV ORF4b is a virulence factor involved in the inflammatory pathology induced in the lungs of mice. PLoS Pathog. 18, e1010834.
  • Enjuanes, L., Sola, I., Zuñiga, S., Honrubia, J. M., Bello-Perez, M., Sanz-Bravo, A., Gonzalez-Miranda, E., Hurtado-Tamayo, J., Requena-Platek, R., Wang, L., Muñoz-Santos, D., Sanchez, C. M., Esteban, A., Ripoll-Gomez, J. 2022. Nature of viruses and pandemics: Coronaviruses. Current Research in Immunology, 3, 151-158.
  • Martínez-González, B., Soria, M. E., Vázquez-Sirvent, L., Ferrer-Orta, C., Lobo-Vega, R., Mínguez, P., de la Fuente, L., Llorens, C., Soriano, B., Ramos-Ruíz, R., Cortón, M., López-Rodríguez, R., García-Crespo, C., Somovilla, P., Durán-Pastor, A., Gallego, I., de Ávila, A. I., Delgado, S., Morán, F., López-Galíndez, C., Gomez, J., Enjuanes, L., Salar-Vidal, L., Esteban-Muñoz, M., Esteban, J., Fernández-Roblas, R., Gadea, I., Ayuso, C., Ruíz-Hornillos, J., Verdaguer, N., Domingo, E., Perales, C. 2022. SARS-CoV-2 Mutant Spectra at Different Depth Levels Reveal an Overwhelming Abundance of Low Frequency Mutations. Pathogens, 11, 662.
  • Du, W., Hurdiss, D. L., Drabek, D., Mykytyn, A. Z., Kaiser, F. K., González-Hernandez, M., Muñoz-Santos, D., Lamers, M. M., van Haperen, R., Li, W., Drulyte, I., Wang, C., Sola, I., Armando, F., Beythien, G., Ciurkiewicz, M., Baumgärtner, W.,  Guilfoyle, K., Smits, T., van der Lee, J., van Kuppeveld, F. J. M., van Amerongen, G.,  Haagmans, B. L., Enjuanes, L., Osterhaus, A. D. M. E., Grosveld, F., Bosch, B. J. 2022. An ACE2-blocking antibody confers broad neutralization and protection against Omicron and other SARS-CoV-2 variants. Immunol. 7, eabp9312.
  • Wong, L. R., Zheng, J., Wilhelmsen, K., Li, K., Ortiz, M. E., Schnicker, N. J., Thurman, A., Pezzulo, A. A., Szachowicz, P. J., Li, P., Pan, R., Klumpp, K., Aswad, F., Rebo, J., Narumiya, S., Murakami, M., Zuniga, S., Sola, I., Enjuanes, L., Meyerholz, D. K., Fortney, K., McCray, P. B., Jr., Perlman, S. 2022. Eicosanoid signaling blockade protects middle-aged mice from severe COVID-19. Nature, 605,146-151.
  • Martínez-González, B., Soria, M. E., Vázquez-Sirvent, L., Ferrer-Orta, C., Lobo-Vega, R., Mínguez, P., de la Fuente, L., Llorens, C., Soriano, B., Ramos, R., Cortón, M., López-Rodríguez, R., García-Crespo, C., Gallego, I., de Ávila, A.I., Gómez, J., Enjuanes, L., Salar-Vidal, L., Esteban, J., Fernandez-Roblas, R., Gadea, I., Ayuso, C., Ruíz-Hornillos, J., Verdaguer, N., Domingo, E., Perales, C., 2022. SARS-CoV-2 Point Mutation and Deletion Spectra and Their Association with Different Disease Outcomes. Microbiol. Spectr. 11, e0022122.
  • Rodriguez-Conde, S., Inman, S., Lindo, V., Amery, L., Tang, A., Okorji-Obike, U., Du, W., Bosch, B. J., Wichgers Schreur, P. J., Kortekaas, J., Sola, I., Enjuanes, L., Kerry, L., Mahal, K., Hulley, M., Daramola, O. 2022. Suitability of transiently expressed antibodies for clinical studies: product quality consistency at different production scales. MAbs 14, 2052228.
  • Morales, L., Oliveros, J. C., Enjuanes, L., Sola, I., 2022. Contribution of host miRNA-223-3p to SARS-CoV-induced lung inflammatory pathology. mBio 13, e0313521.
  • Varona, J. F., Landete, P., Lopez-Martin, J. A., Estrada, V., Paredes, R., Guisado-Vasco, P., Fernandez de Orueta, L., Torralba, M., Fortun, J., Vates, R., Barberan, J., Clotet, B., Ancochea, J., Carnevali, D., Cabello, N., Porras, L., Gijon, P., Monereo, A., Abad, D., Zuñiga, S., Sola, I., Rodon, J., Vergara-Alert, J., Izquierdo-Useros, N., Fudio, S., Pontes, M. J., de Rivas, B., Giron de Velasco, P., Nieto, A., Gomez, J., Aviles, P., Lubomirov, R., Belgrano, A., Sopesen, B., White, K.M., Rosales, R., Yildiz, S., Reuschl, A.K., Thorne, L.G., Jolly, C., Towers, G.J., Zuliani-Alvarez, L., Bouhaddou, M., Obernier, K., McGovern, B.L., Rodriguez, M.L., Enjuanes, L., Fernandez-Sousa, J. M., Krogan, N.J., Jimeno, J. M., Garcia-Sastre, A., 2022. Preclinical and randomized phase I studies of plitidepsin in adults hospitalized with COVID-19. Life Sci. Alliance 5, e202101200
  • Ujike, M., Etoh, Y., Urushiyama, N., Taguchi, F., Asanuma, H., Enjuanes, L., Kamitani, W., 2021. Reverse Genetics with a Full-length Infectious cDNA Clone of Bovine Torovirus. Virol. 96, e0156121.
  • Malicoat, J., Manivasagam, S., Zuñiga, S., Sola, I., McCabe, D., Rong, L., Perlman, S., Enjuanes, L., Manicassamy, B., 2021. Development of a single-cycle infectious SARS-CoV-2 virus replicon particle system for use in BSL2 laboratories. Virol. 96, e0183721.
  • Gutierrez-Álvarez, J., Honrubia, J. M., Sanz-Bravo, A., Gonzalez-Miranda, E., Fernández-Delgado, R., Rejas, M. T., Zúñiga, S., Sola, I., Enjuanes, L. 2021. Middle East respiratory syndrome coronavirus vaccine based on a propagation-defective RNA replicon elicited sterilizing immunity in mice. Natl. Acad. Sci. USA 118, e2111075118.
  • Wang L, Sola I, Enjuanes L, Zuniga S. 2021. MOV10 Helicase interacts with coronavirus nucleocapsid protein and has antiviral activity. mBio 12, e01316-21.
  • Calisher, C. H., Carroll, D., Colwell, R., Corley, R. B., Daszak, P., Drosten, C., Enjuanes, L., Farrar, J., Field, H., Golding, J., Gorbalenya, A. E., Haagmans, B., Hughes, J. M., Keusch, G. T., Lam, S. K., Lubroth, J., Mackenzie, J. S., Madoff, L., Mazet, J. K., Perlman, S. M., Poon, L., Saif, L., Subbarao, K., Turner, M., 2021. Science, not speculation, is essential to determine how SARS-CoV-2 reached humans. Lancet 398, 209-211.
  • Castaño-Rodriguez, C., Honrubia, J. M., Gutiérrez-Álvarez, J., Sola, I., Enjuanes, L., 2021. Viral PDZ binding motifs influence cell behavior through the interaction with cellular proteins containing PDZ domains. Methods Mol. Biol. 2256, 217-236.
  • Ruano-Gallego, D., García-Villadangos, M., Moreno-Paz, M., Gómez-Elvira, J., Postigo, M., Simón-Sacristán, M., Reyburn, H.T., Carolis, C., Rodrigo, N., Codeseira, Y.B., Rueda, P., Zúñiga, S., Enjuanes, L., Parro, V., 2021. A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported. Microbial Biotechnology 14, 1228-1236.
  • Wang, C., van Haperen, R., Gutierrez-Alvarez, J., Li, W., Okba, N.M.A., Albulescu, I., Widjaja, I., van Dieren, B., Fernandez-Delgado, R., Sola, I., Hurdiss, D.L., Daramola, O., Grosveld, F., van Kuppeveld, F.J.M., Haagmans, B.L., Enjuanes, L., Drabek, D., Bosch, B.J., 2021. A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies. Commun. 12, 1715.
  • Gutiérrez-Álvarez, J., Honrubia, J. M., Fernández-Delgado, R., Wang, L., Castaño-Rodríguez, C., Zúñiga, S., Sola, I., and Enjuanes, L. 2021. Genetically engineered live-attenuated MERS-CoV viruses conferred full protection against lethal infection. mBio 12, e00103-21.
  • Gutierrez-Alvarez, J., Wang, L., Fernandez-Delgado, R., Li, K., McCray, P.B., Jr., Perlman, S., Sola, I., Zuniga, S., Enjuanes, L., 2021. Middle East Respiratory Syndrome Coronavirus Gene 5 Modulates Pathogenesis in Mice. J. Virol. 95, e01172-01120.
  • Gorbalenya, A. E., Baker, S. C., Baric, R. S., de Groot, R. J., Drosten, C., Gulyaeva, A. A., Haagmans, B. L., Lauber, C., Leontovich, A. M., Neuman, B. W., Penzar, D., Perlman, S., Poon, L. L. M., Samborskiy, D. V., Sidorov, I. A., Sola, I., Ziebuhr, J. 2020. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2. Coronaviridae Study Group of the International Committee on Taxonomy of Viruses. Microbio. 5, 536–544
  • Diez, J. M., Romero, C., Vergara-Alert, J., Belló-Perez, M., Rodon, J., Honrubia, J. M., Segalés, J., Sola, I., Enjuanes, L., Gajardo, R. 2020. Cross-neutralization activity against SARS-CoV-2 is present in currently available intravenous immunoglobulins. Immunotherapy, 12, 1247-1255.
  • Enjuanes, L., Sola, I., Zuñiga, S., 2020. Novel human pathogenic coronavirus: SARS-CoV-2. FEMS, 1-5.
  • Calisher, C., Carroll, D., Colwell, R., Corley, R. B., Daszak, P., Drosten, C., Enjuanes, L., Farrar, J., Field, H., Golding, J., Gorbalenya, A., Haagmans, B., Hughes, J. M., Karesh, W. B., Keusch, G. T., Lam, S. K., Lubroth, J., Mackenzie, J. S., Madoff, L., Mazet, J., Palese, P., Perlman, S., Poon, L., Roizman, B., Saif, L., Subbarao, K., Turner, M., Less, S. 2020. Statement in support of the scientists, public health professionals, and medical professionals of China combatting COVID-19. The Lancet, Correspondence 395, e42-e43.
  • Sanchez, C. M., Pascual-Iglesias, A., Sola, I., Zuñiga, S., Enjuanes, L., 2019. Minimum determinants of transmissible gastroenteritis virus enteric tropism are located in the N-terminus of spike protein. Pathogens 9, 2.
  • Pascual-Iglesias, A., Sanchez, C. M., Penzes, Z., Sola, I., Enjuanes, L., Zuniga, S., 2019. Recombinant chimeric transmissible gastroenteritis virus (TGEV) – porcine epidemic diarrhea virus (PEDV) virus provides protection against virulent PEDV. Viruses 11, 682.
  • Siu, K. L., Yuen, K. S., Castano-Rodriguez, C., Ye, Z. W., Yeung, M. L., Fung, S. Y., Yuan, S., Chan, C. P., Yuen, K. Y., Enjuanes, L., Jin, D. Y., 2019. Severe acute respiratory syndrome coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC. FASEB J. 33, fj201802418R.
  • Widjaja, I., Wang, C., van Haperen, R., Gutierrez-Alvarez, J., van Dieren, B., Okba, N. M. A., Raj, V. S., Li, W., Fernandez-Delgado, R., Grosveld, F. G., van Kuppeveld, F. J. M., Haagmans, B. L., Enjuanes, L., Drabek, D., Bosch, B. J., 2019. Towards a solution to MERS: protective human monoclonal antibodies targeting different domains and functions of the MERS-coronavirus spike glycoprotein. Microbes Infect. 8, 516-530.
  • Suzuki, T., Terada, Y., Enjuanes, L., Ohashi, S., Kamitani, W., 2018. S1 subunit of spike protein from a current highly virulent porcine epidemic diarrhea virus is an important determinant of virulence in piglets. Viruses 10, 467.
  • Letko, M., Miazgowicz, K., McMinn, R., Seifert, S. N., Sola, I., Enjuanes, L., Carmody, A., van Doremalen, N., Munster, V., 2018. Adaptive evolution of MERS-CoV to species variation in DPP4. Cell Rep. 24, 1730-1737.
  • Stalin Raj, V., Okba, N. M. A., Gutierrez-Alvarez, J., Drabek, D., van Dieren, B., Widagdo, W., Lamers, M. M., Widjaja, I., Fernandez-Delgado, R., Sola, I., Bensaid, A., Koopmans, M. P., Segales, J., Osterhaus, A., Bosch, B. J., Enjuanes, L., Haagmans, B. L., 2018. Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection. Adv. 4, eaas9667.
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  • Fernandez, L. A., Sola, I., Enjuanes, L., de Lorenzo, V. 2000. Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system. Environ. Microbiol. 66, 5024-5029
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  • Sanchez, C. M., Izeta, A., Sanchez-Morgado, J. M., Alonso, S., Sola, I., Balasch, M., Plana-Duran, J., Enjuanes, L. 1999. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. Virol. 73, 7607-7618.
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