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- Zuñiga, S., Sola, I., Moreno, J. L., Sabella, P., Plana-Duran, J., and Enjuanes, L. 2007. Coronavirus nucleocapsid protein is an RNA chaperone. Virology 357, 215-227.
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- DeDiego, M. L., Alvarez, E., Almazan, F., Rejas, M. T., Lamirande, E., Roberts, A., Shieh, W. J., Zaki. S. R., Subbarao, K., and Enjuanes, L. 2007. A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo. Virol. 81, 1701-1713.
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- Alamillo, J. M., Monger, W., Sola, I., Garcia, B., Perrin, Y. Bestagno, M., Burrone, O., Sabella, P., Plana-Duran, J., Enjuanes, L., Lomonossoff, G. P. and Garcia, J. A. 2006. Use of virus vectors for the expression in plants of active full-length and single chain anti-coronavirus antibodies. Biotechnol. J. 1, 1103-1111.
- Enjuanes, L., Almazan, F., Sola, I., and Zuñiga, S. 2006. Biochemical aspects of coronavirus replication and virus host-interaction. Rev. Microbiol. 60, 211-230.
- Gorbalenya, A. E., Enjuanes, L., Ziebuhr, J., and Snijder, E. J. 2006. Nidovirales: Evolving the largest RNA virus genome. Virus Res. 117, 17-37.
- Galan, C., Almazan, F., and Enjuanes, L. 2006. Differential role of N-terminal polyprotein processing in coronavirus genome replication and minigenome amplification. Adv. Exp. Med. Biol. 581, 79-83.
- Almazan, F., DeDiego, M. L., Galan, C., Alvarez, E., and Enjuanes, L. 2006. Identification of essential genes as a strategy to select a SARS candidate vaccine using a SARS-CoV infectious cDNA. Adv. Exp. Med. Biol. 581, 579-583.
- Zuñiga, S., Sola. I., Moreno, J. L., Alonso, S., and Enjuanes, L. 2006. Regulation of coronavirus transcription: viral and cellular proteins interacting with transcription-regulating sequences. Adv. Exp. Med. Biol. 581, 31-35.
- Enjuanes, L., Almazan, F., Sola. I., Zuñiga, S., Alvarez, E., Reguera, J., and Capiscol, C. 2006. Biochemical aspects of coronavirus replication. Adv. Exp. Med. Biol. 581, 13-24.
- Monger, W., Alamillo, J. M., Sola, I., Perrin, Y. Bestagno, M., Burrone, O., Sabella, P., Plana-Duran, J., Enjuanes, L., Garcia, J. A., and Lomonossoff, G. P. 2006. An antibody derivative expressed from viral vectors passively immunizes pigs against transmissible gastroenteritis virus infection when supplied orally in crude plant extracts. Plant Biotechnol. J. 4, 623-631.
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- Galan, C., Enjuanes, L., and Almazan, F. 2005. A point mutation within the replicase gene differentially affects coronavirus genome versus minigenome replication. J. Virol. 79, 15016-15026.
- Calvo, E., Escors D., Lopez, J. A., Gonzalez, J. M., Alvarez, A., Arza, E., Enjuanes, L. 2005. Phosphorylation of transmissible gastroenteritis coronavirus nucleocapsid protein and subcellular localization in infected cells. J. Gen Virol. 86, 2255-2267.
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- Sola, I., Moreno, J. L., Zuñiga, S., Enjuanes, L. 2005. Role of nucleotides immediately flanking the transcription-regulating sequence core in coronavirus subgenomic mRNA synthesis. J. Virol. 79, 2506-2516.
- Enjuanes, L., Sola, I., Alonso, S., Escors, D., Zuñiga, S. 2005. Coronavirus reverse genetics and development of vectors for gene expression. Top. Microbiol. & Immunol. 287, 161-197.
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- Almazan, F., Galan, C., Enjuanes, L. 2004. The nucleoprotein is required for efficient coronavirus genome replication. Virol. 78, 12683-12688.
- Escors, D., Capiscol, C., Enjuanes, E. 2004. Immunopurification applied to the study of virus protein composition and encapsidation. J. Virol. Methods. 119, 57-64.
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- Bailey, M., Haweson, K., Jones, P., Miller, B., Sola, I., Enjuanes, L., Stokes, C. R. 2004. Effect of infection with transmissible gastroenteritis virus on concomitant immune responses to dietary and injected antigens. Clin. Diagn. Lab. Immun. 11, 337-343.
- Zuñiga, S., Sola, I., Alonso, S., Enjuanes, L. 2004. Sequence motifs involved in the regulation of discontinuous coronavirus subgenomic RNA synthesis. J. Virol. 78, 980-994.
- Gonzalez, J. M., Gomez-Puertas, P., Cavanagh, D., Gorbalenya, A. E., Enjuanes. 2003. A comparative sequence analysis to revise the current taxonomuy of the family Coronaviridae. Arch. Virol. 148, 2207-2235.
- Escors, D., Izeta, A., Capiscol, C., Enjuanes, L. 2003. Transmisible gastroenteritis coronavirus packaging signal is located at the 5’ end of the virus genome. Virol. 77, 7890-7902.
- Holmes, K. V., Enjuanes, L. 2003. The SARS coronavirus: a postgenomic era. Science 300, 1377-1378.
- Sola, I., Alonso, S., Zuñiga, S., Balach, M., Plana-Duran, J. y Enjuanes, L. 2003. Engineering transmissible gastroenteritis virus genome as an expression vector inducing latogenic immunity. J. Virol. 77, 4357-4369.
- Ortego, J., Sola I., Almazan F., Ceriani J. E., Riquelme C., Balasch M., Plana J., and Enjuanes, L. 2003. Transmissible gastroenteritis coronavirus gene 7 is not essential, but affects in vivo replication and virulence. Virology 308, 13-22.
- Ortego, J., Escors, D., H. Laude y L. Enjuanes. 2002. Generation of a replication-competent, propagation-deficient virus vector based on transmissible gastroenteritis coronavirus genome. Virol. 76, 11518-11529.
- Riquelme, C., Escors, D., Ortego, J., Sanchez, C. M., Uzelac-Keserovic, B., Apostolov, K., Enjuanes, L. 2002. Nature of the virus associated with endemic Balkan nephropaty. Emerg. Dis. 8, 869-870.
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- Gonzalez, J.M., Penzes, Z., Almazan, F., Calvo, E., Enjuanes, L. 2002. Stabilization of a full-length infectious cDNA clone of transmissible gastroenteritis coronavirus by insertion of an intron. J. Virol. 76, 4655-4661.
- Alonso, S., A. Izeta, I. Sola y L. Enjuanes. Tanscription regulatory sequences and mRNA expression levels in the coronavirus transmissible gastroenteritis virus. J. Virol. 76, 1293-1308.
- Alonso, S., I. Sola, J. P. Teifke, I. Reimann, A. Izeta, M. Balach, J. Plana-Duran, R. J. M. Moormann y L. Enjuanes. 2002. In vitro and in vivo expression of foreign genes by transmissible gastroenteritis coronavirus-derived minigenomes. Virol. 83, 567-579.
- Escors, D., Camafeita, E., J. Ortego, H. Laude y L. Enjuanes. 2001. Organization of two transmissible gastroenteritis coronavirus membrane protein topologies within the virion and core. Virol. 75, 12228-12240.
- Enjuanes, L., Sola, I., Almazan, F., Izeta, A., Gonzalez, J.M., Alonso, S., Sanchez-Morgado, J. M., Ortego, J., Escors, D., Calvo, E., Riquelme, C., Sanchez, C. M. 2001. Coronavirus derived expression systems. J. Biotechnol. 88, 183-204
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- Gonzalez, J. M., Almazan, F., Penzes, Z.,Calvo, E., Enjuanes, L. 2001. Cloning of a transmissible gastroenteritis coronavirus full-length cDNA. Adv. Exp. Med. 494, 533-536.
- Escors, D., Ortego, J., Enjuanes, L. 2001. The membrane M protein of the transmissible gastroenteritis coronavirus binds to the internal core through the carboxy-terminus. Exp. Med. Biol. 494, 589-594.
- Almazan, F., Gonzalez, J. M., Penzes, Z., Izeta, A., Calvo, E., Enjuanes, L. 2001. A strategy for the generation of an infectious transmissible gastroenteritis coronavirus from cloned cDNA. Adv. Exp. Med. Biol. 494, 261-266.
- Sola, I., Alonso, S., Sanchez, C. M., Sanchez-Morgado, J. M., Enjuanes, L. 2000. Expression of transcriptional units using transmissible gastroenteritis coronavirus derived minigenomes and full-length cDNA clones. Exp. Med. Biol. 494, 447-452.
- Enjuanes, L., Sola, I., Almazan, F., Izeta, A., Gonzalez, J. M., Alonso, S. 2000. Coronavirus derived expression systems. Adv. Exp. Med. Biol. 494, 309-322.
- Escors, D., Ortego, J., Laude, H., Enjuanes, L. 2000. The membrane M protein carboxy terminus binds to transmissible gastroenteritis coronavirus core and contributes to core stability. Virol. 75, 1312-1324.
- Fernandez, L. A., Sola, I., Enjuanes, L., de Lorenzo, V. 2000. Specific secretion of active single-chain Fv antibodies into the supernatants of Escherichia coli cultures by use of the hemolysin system. Environ. Microbiol. 66, 5024-5029
- Almazan, F., Gonzalez, J. M., Penzes, Z., Izeta, A., Calvo, E., Plana-Duran, J., Enjuanes, L. 2000. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Nat. Acad. Sci. USA. 97, 5516-5521.
- Krempl, C., Ballesteros, M. L., Zimmer, G., Enjuanes, L., Klenk, H.-D., Herrler, G. 2000. Characterization of the sialic acid binding activity of transmissible gastroenteritis coronavirus by analysis of hemagglutination-deficient mutants. Gen. Virol. 81, 489-496.
- Sanchez, C. M., Izeta, A., Sanchez-Morgado, J. M., Alonso, S., Sola, I., Balasch, M., Plana-Duran, J., Enjuanes, L. 1999. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. Virol. 73, 7607-7618.
- Enjuanes, L., Sola, I., Sanchez-Morgado, J. M., Izeta, A., Gonzalez, J.M., Alonso, S., Escors, D., Sanchez, C.M. 1999. Interference with virus and bacteria replication by the tissue specific expression of antibodies and interfering molecules. Exp. Med. Biol. 473, 31-45.
- Izeta, A., Smerdou, C., Alonso, S., Penzes, Z., Mendez, A., Plana-Duran, J., Enjuanes, L. 1998. Replication and packaging of transmissible gastroenteritis coronavirus derived synthetic minigenomes. Virol. 73, 1535-1545.
- Krempl, C., Ballesteros, M. L., Enjuanes, L., Herrler, G. 1998. Isolation of hemagglutination-defective mutants for the analysis of the sialic acid binding activity of transmissible gastroenteritis virus. Exp. Med. Biol. 440, 563-568.
- Risco, C., Anton, I., Muntion, M., Gonzalez, J. M., Carrascosa, J. L., Enjuanes, L. 1998. Structure and intracellular assembly of the transmissible gastroenteritis coronavirus. Adv. Exp. Med. Biol. 440, 341-346.
- Penzes, Z., Gonzalez, J., Izeta, A., Muntion, M., Enjuanes, L. 1998. Progress towards the construction of a transmissible gastroenteritis coronavirus self-replicating RNA using a two-layer expression system. Exp. Med. Biol. 440, 319-326.
- Izeta, A., Sanchez, C. M., Smerdou, C., Mendez, A., Alonso, S., Balasch, M., Plana-Duran, J., Enjuanes, L. 1998. The spike protein of transmissible gastroenteritis coronavirus controls the tropism of pseudorecombinant virions engineered using synthetic minigenomes. Exp. Med. Biol. 440, 207-214.
- Castilla, J., Sola, I., Pintado, B., Sanchez, J. M., Enjuanes, L. 1998. Lactogenic immunity in transgenic mice producing recombinant antibodies neutralizing coronavirus. Exp. Med. Biol. 440, 675-686.
- Sola, I., Castilla, J., Enjuanes, L. 1998. Interference of coronavirus infection by expression of IgG or IgA virus neutralizing antibodies. Exp. Med. Biol. 440, 665-674.
- Castilla, J., Pintado, B., Sola, I., Sanchez-Morgado, J. M., and Enjuanes, L. 1998. Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk. Nature Biotech. 16, 349-354
- Risco, C., Muntion, M., Enjuanes, L., Carrascosa J. L. 1998. Two types of viral-related particles are found during transmissible gastroenteritis virus morphogenesis. Virol. 72: 4022-4031.
- Sola, I., Castilla, J., Pintado, B., Sanchez-Morgado, J. M., Whitelaw, B., Clark, J., and, Enjuanes, L. 1998. Transgenic mice secreting coronavirus neutralizing antibodies into the milk. J. Virol. 72, 3762-3772
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- Cavanagh, D., Brian, D.A., Briton, P., Enjuanes, L., Horzinek, M. C., Lai, M.M.C., Laude, H., Plagemann, P.G.W., Siddell, S, Spaan, Talbot, P.J. 1997. Nidovirales: a new order comprising Coronaviridae and Arch. Virol. 142, 629-635.
- Ballesteros, M. L., Sanchez, C. M., Enjuanes, L. 1997. Two amino acid changes at the N-terminus of transmissible gastroenteritis coronavirus spike protein result in the loss of enteric tropism. Virology 227, 378-388.
- Anton, I., Gonzalez, S., Bullido, M. J., Corsin, M., Risco, C., Langeveld, J. P. M., Enjuanes, L. 1996. Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies. Res. 46, 111-124.
- Schultze, B., Krempl, C., Ballesteros, M. L., Shaw, L. Schauer, R., Enjuanes, L., and Herrler, G. 1996. Transmissible gastroenteritis coronavirus, but not the related porcine respiratory coronavirus, has a sialic acid (N-glycoloylneuraminic acid) binding activity. Virol. 70, 5634-5637.
- Callebaut, P., Enjuanes, L. and, Pensaert, M. 1996. An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus immunogenic in swine. J. Gen Virol. 77, 309-313.
- Mendez, A., Smerdou, C. Izeta, A., Gebauer, F., and Enjuanes, L. 1996. Molecular characterization of transmissible gastroenteritis coronavirus defective interfering genomes: Packaging and heterogeneity. Virology 217, 495-507.
- Smerdou, C., Anton, I. M., Plana, J., Curtiss, R., and Enjuanes, L. 1996. A continuous epitope from transmissible gastroenteritis virus S protein fused to E. coli heat-labile toxin B subunit expressed by attenuated Salmonella induces serum and secretory immunity. Virus Res. 41, 1-9.
- Smerdou, C., Urniza, A., Curtiss, R., and Enjuanes, L. 1996. Characterization of transmissible gastroenteritis coronavirus S protein expression products in avirulent S. typhimurium Dcya ∆crp: persistence, stability and immune response in swine. Veterinary Microbiol. 48, 87-100.
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- Torres, J. M., Covadonga, A., Ortega, A., Mittal, S., Graham, F., and Enjuanes, L. 1996. Tropism of human adenovirus Ad5 based vectors in swine and their use in protection against transmissible gastroenteritis coronavirus. Virol. 70, 3770-3780.
- Torres, J. M., Sanchez, C. M., Suñe, C., Smerdou, C., Preveck, L., Graham, F., Enjuanes, L. 1995. Induction of antibodies protecting against transmissible gastroenteritis coronavirus (TGEV) by recombinant adenovirus expressing TGEV spike protein. Virology 213, 503-516.
- Risco, C., Anton, I. M., Suñe, C., Pedregosa, A. M., Martin-Alonso, J. M., Parra, F., Carrascosa, J. L., and Enjuanes, L. 1995. Membrane protein molecules of Transmissible gastroenteritis coronavirus also expose the carboxy-terminal region on the external surface of the virion. J. Virol. 69, 5269-5277.
- Enjuanes, L., Smerdou, C., Sanchez, C. M., Suñe, C., Kelly, S., Curtiss III, Roy, and Torres, J. M. 1995. Induction of transmissible gastroenteritis coronavirus specific immune responses using vectors with enteric tropism. Exp. Med. Biol. 380, 1535-1541.
- Schultze, B., Enjuanes, and L.Herrler, G. 1995. Analysis of the sialic acid-binding activity of transmissible gastroenteritis virus. Adv. Exp. Med. Biol. 380, 367-370.
- Ballesteros, M. L., Sanchez, C. M., Martin-Caballero, J., and Enjuanes, L. 1995. Molecular bases of tropism in the PUR46 cluster of Transmissible gastroenteritis coronaviruses. Adv. Exp. Med. Biol. 380, 557-562.
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- Enjuanes, L., Smerdou, C., Castilla, J., Anton, Torres, J. M., I. M., Sola, I., Golvano, J., Sanchez, J. M., and Pintado, B. 1995. Development of protection against coronavirus induced diseases. Adv. Exp. Med. Biol. 380, 197-212.
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- Cavanagh, D., Brian, D. A., Brinton, M. A., Enjuanes, L., Holmes, K. V., Horzinek, M. C., Lai, M. M. C., Laude, H., Plagemann, P. G. W., Siddell, S. G., Spaan, W. J. M., Taguchi, F., Talbot, P. J. 1993. The Coronaviridae now comprises two genera, Coronavirus and Torovirus: Report of the Coronaviridae Study group. Adv. Exp. Med. Biol. 342, 255-257.
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